Cases of affinity chromatography media
Affinity chromatography is a chromatographic method that separates substances based on specific interactions between biological molecules, such as enzyme substrate binding, receptor ligand binding, antibody antigen binding, etc. This type of binding is both specific and reversible. The goal of protein purification is achieved through this reversible binding and separation.
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Cases of ion exchange chromatography media
Ion exchange chromatography is a chromatographic technique that separates different biological molecules based on the differences in their charge properties and quantities. Due to the fact that most biomolecules contain acidic or alkaline groups, and their charged properties and quantity can be changed by adjusting the pH of the buffer solution, biomolecules can be separated by changing the ion strength or pH in the mobile phase after being combined with anionic exchange media with opposite charges or cation exchange media, allowing weak binding to elute first and strong binding to elute later.
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Case study of hydrophobic interaction chromatography medium
Hydrophobic interaction chromatography is a commonly used chromatography method that separates proteins or peptides based on the difference in molecular surface hydrophobicity. Proteins are adsorbed on hydrophobic groups in the hydrophobic chromatography medium under high salt conditions. When the salt concentration is reduced, different proteins are eluted in order of hydrophobicity from weak to strong, thus achieving the purpose of separation and purification. In hydrophobic chromatography, binding force is mainly affected by ligand type, buffer salt concentration, chromatography temperature, pH, and other factors. Sanji Technology's hydrophobic interaction chromatography medium is a type of chromatography medium that uses Sanarose 4FF and 6FF as matrices and phenyl, octyl, and butyl as ligands. It has the advantages of high hardness, fast flow rate, strong selectivity, high activity recovery rate, and no environmental pollution. It can withstand strong acids and bases, and can be cleaned in place and sterilized at high temperatures. Compared with reverse phase chromatography medium, hydrophobic chromatography has lower ligand concentration and mild elution conditions, which can better maintain the biological activity of the sample.
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Case of gel filtration chromatography medium
Gel filtration chromatography, also known as molecular sieve chromatography or volume exclusion chromatography, is a chromatography method that separates substances according to the size and shape of biological molecules. Its chromatography medium is a porous network structure, and the pore size of each medium has a certain range. Select a suitable separation range to achieve the purpose of separating proteins with different molecular weights.
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