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Introduction of his tag and sharing of application cases

Example 1: Ni NTA sanarose 6ff purified protein A

Chromatographic column information: 5ml pre installed column
Buffer system:

Binding Buffer：50mM Tris-HCl，150mM NaCl，10mM Imidazole，pH 8.0

Wash Buffer：50mM Tris-HCl，150mM NaCl，50mM Imidazole，pH 8.0

Elution Buffer：50mM Tris-HCl，150mM NaCl，200mM Imidazole，pH 8.0

Purification scheme:
► balance: binding buffer balance 5cv, flow rate 2ml/min;
► sample loading: flow rate 2ml/min, sample loading volume 50ml;
► impurity washing: wash buffer 3cv, flow rate 2ml/min;
► elution: elution buffer elutes and collects the target protein;
Electrophoresis detection of purification results:

Fig. 1 electrophoresis results of Ni NTA sanarose 6ff purified protein A

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Example 2: Ni IDA sanarose 6ff purified protein G

Chromatographic column information: 5ml pre installed column
Buffer system:
Binding buffer: 50mm Tris HCl, 500mm NaCl, 10mm imidazole, pH 9.0
Wash buffer: 50mm Tris HCl, 150mm NaCl, 50mm imidazole, pH 9.0
Elution buffer: 50mm Tris HCl, 150mm NaCl, 200~500mm imidazole, pH 9.0
Purification scheme:
► balance: binding buffer balance 5cv, flow rate 2ml/min;
► sample loading: flow rate 2ml/min, sample loading volume 50ml;
► impurity washing: wash buffer 3cv, flow rate 2ml/min;
► elution: elution buffer elutes and collects the target protein;
Electrophoresis detection of purification results:

Fig. 2 electrophoresis results of purified protein A of Ni IDA sanarose 6ff

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