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SP Sanarose 6FF-Purification and development of lactoferrin

Purification and development of lactoferrin
1. Purification process

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2. purification result example sp sanarose 6ff

1）Purification operation1) Regeneration: 1m NaOH, flow rate 3ml/min;
2) Cleaning: pure water, flow rate 3ml/min;
3) Flushing: eluent, flow rate 3ml/min;
4) Balance: balance solution, flow rate 3ml/min;
5) Loading: pump a directly loads about 500ml of sample at a flow rate of 3ml/min;
6) Rebalance: equilibrium solution, flow rate 3ml/min;
7) Elution: eluent, flow rate 3ml/min, collect elution peak;
8) Elution: eluent, flow rate 3ml/min, collect elution peak;
9) Alkali washing: alkali washing solution, flow rate 3ml/min, collect alkali washing peak;
10) Cleaning: pure water, flow rate 3ml/min;
2) Purification results:

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图二乳铁蛋白纯化洗脱曲线（备注：100%B洗脱为乳铁蛋白）

 

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图三乳铁蛋白纯化原液电泳检测

 

3）conclusion

The purity of whole milk powder purified by spsanarose 6ff was more than 95%.