SP Sanarose HP
Classify :
Cation Exchange Chromatography Medium
Keyword：
SANJI TECH
Product Features
Product Details
Product Introduction
The ion exchange chromatography medium SP Sanarose HP is a high-resolution strong cation exchange medium with sulfopropyl as the functional ligand. Compared with SP Sanarose 6FF, the particle size range is narrower. It retains the natural hydrophilicity and porous structure of agarose, has good biocompatibility, stable physicochemical properties, can withstand strong acids and alkalis, and can be cleaned in place and sterilized at high temperatures.
Ion exchange chromatography technology achieves separation based on the difference in net charges on the surface of biological molecules. SP Sanarose HP is widely used in laboratories and downstream stages of biotechnology. Its operation is simple, easy to regenerate, selectivity is good, recovery rate is high, non-specific adsorption is low, and binding load is high. It has good effects in the purification fields of proteins, viruses, nucleic acids, and peptides. In typical three part purification strategies, it is mostly used for capture and moderate purification.
Technical Parameter
|
Media |
SP Sanarose HP |
|
Scaffold |
Highly cross-linked 6% agarose, high resolution |
|
Media Type |
Strong cation |
|
Functional Group |
Sulfopropyl |
|
Dynamic binding capacity |
55mg RNase/ml medium |
|
Average particle size |
34μm |
|
Pressure resistant |
0.3Mpa |
|
Recommended flow rates |
<150cm/h (adjusted according to column size) |
|
Operating pH range |
4-13 |
|
Chemical stability |
Commonly used ion exchange buffer, 1M sodium hydroxide, 1M acetic acid, 70% ethanol, 30% isopropyl alcohol |
|
pH stability |
Long term: 4-13; short term: 3-14 |
|
Save |
20% ethanol containing 0.2M sodium acetate, 4-30℃ |
|
Apply |
Intermediate purification and fine purification stages |
ordering information
filler
|
Product Name |
Article number |
Specifications |
|
SP Sanarose HP |
NSH00100 |
100ml |
|
NSH00250 |
250ml |
|
|
NSH00500 |
500ml |
|
|
NSH01000 |
1L |
prepacked column
|
Product Name |
Article number |
Specifications |
|
SP Sanarose HP |
NSHYZ001 |
1ml(7*25) |
|
NSHYZ05A |
5ml(16*25) |
|
|
NSHYZ05X |
5ml(8*100) |
Contact Us
About us
Address: 5th floor, building 12, Xiamen biomedical collaborative innovation and entrepreneurship center, 71 HouXiang Road, Haicang District, Xiamen