SP Sanarose 6FF
Classify :
Cation Exchange Chromatography Medium
Keyword：
SANJI TECH
Product Features
Product Details
Product Introduction
The ion exchange chromatography medium SP Sanarose 6FF is a strong cation exchange chromatography medium using Sanarose 6FF as the matrix and sulfopropyl as the functional ligand. It retains the natural hydrophilicity and porous structure of agarose, has good biocompatibility, stable physicochemical properties, can withstand strong acids and alkalis, and can be cleaned in place and sterilized at high temperatures. Compared with weak cation exchange chromatography medium, its fully protonated pH range (working pH range) is slightly wider.
Ion exchange chromatography technology achieves separation based on the difference in net charge on the surface of biomolecules. SP Sanarose 6FF is widely used in laboratories and downstream stages of biotechnology. It is easy to operate, easy to regenerate, has good selectivity, high recovery rate, low non-specific adsorption, and high binding capacity. It has good effects in purification fields such as proteins, viruses, nucleic acids, and peptides; Among the typical three part purification strategies, they are mostly used for capture and moderate purification.
Technical Parameter
|
Media |
SP Sanarose 6FF |
|
Scaffold |
Highly cross-linked 6% agarose, high flow rate |
|
Media type |
Strong cation |
|
Functional group |
Sulfopropyl |
|
Dynamic combined loading |
70mg RNase/ml medium |
|
Average particle size |
90μm |
|
Pressure resistant |
0.3Mpa |
|
Recommended flow rate |
<300cm/h (adjusted according to column size) |
|
Working pH range |
4-13 |
|
Chemical stability |
Commonly used ion exchange buffer, 1M sodium hydroxide, 6M guanidine hydrochloride, 70% Ethanol, 30% isopropyl alcohol |
|
pH stability |
Long term: 3~14 Short term: 4~13 |
|
Save |
20% ethanol containing 0.2M sodium acetate, 4~30℃ |
|
Apply |
The entire stage of purification |
ordering information
|
Products Name |
No. |
Specification |
|
SP Sanarose 6FF |
NSB00100 |
100ml |
|
NSB00250 |
250ml |
|
|
NSB00500 |
500ml |
|
|
NSB01000 |
1L |
prepacked column
| Products Name |
No. |
Specification |
|
SP Sanarose 6FF |
NSYZ0001 |
1ml（7*25） |
|
NSYZ005A |
5ml（16*25） |
|
|
NSYZ005X |
5ml（8*100） |
Contact Us
About us
Address: 5th floor, building 12, Xiamen biomedical collaborative innovation and entrepreneurship center, 71 HouXiang Road, Haicang District, Xiamen