Sanarose 6FF
Classify :
Agarose Base
Keyword：
SANJI TECH
Product Features
Product Details
Product Introduction
Sanarose 6FF gel filter media are respectively made of Sanarose 6B and 2,3-dibromopropanol through multi-step cross-linking. The degree of cross-linking is high, and it has certain mechanical strength. Compared with other agarose gel filter media, its advantages are mainly reflected in: when maintaining good chromatographic performance conditions, its flow rate and pressure resistance are significantly improved, the sample processing volume is large, the repeatability between batches is good, and it can withstand the complex and changing production environment of pharmaceutical proteins.
Gel filtration chromatography realizes separation based on the difference of molecular size and shape. Sanarose 6FF is widely used in laboratories and downstream stages of bioengineering, and can be used to remove the foreign proteins in viruses and nucleic acids, and remove the foreign proteins in culture medium in the purification of viral vaccines; It can also serve as a base for chemical modification, coupling functional ligands, and preparing other types of chromatography media.
Technical Parameter
|
Media |
Sanarose 6FF |
|
Particle size range(μm) |
45~165 |
|
Average particle size(μm) |
90 |
|
Separate range |
10kd ~ 1×103kd (linear) |
|
Run flow rate |
<300cm/h |
|
Tolerance to stress |
0.3Mpa |
|
pH tolerant |
Long term: 2~12; short term 2~14 |
|
Chemical stability |
Tolerant to commonly used gel filtration solutions; resistant to 8M urea, 6M guanidine hydrochloride, 70% ethanol, 2M sodium hydroxide, 30% isopropyl alcohol, 1% SDS, etc. |
|
Save |
20% ethanol, 4~30℃, preferably refrigerated, do not freeze, and replace the storage solution regularly. |
|
Cleaning and Regeneration |
It can be soaked in 0.5M NaOH solution for 1 to 2 hours to achieve sterilization effect; 2M NaCl, 1M NaOH, 0.5M acetic acid, 70% ethanol, etc. can be selected according to the type of pollutants. Flush multiple column volumes with 30% isopropyl alcohol, 1% nonionic detergent, ultrapure water, etc. to achieve the effect of cleaning and regeneration. |
Product ordering information
Filler
|
Product Name |
Article number |
Specifications |
|
Sanarose 6FF |
NFC00100 |
100mL |
|
NFC00500 |
500mL |
|
|
NFC01000 |
1L |
Contact Us
About us
Address: 5th floor, building 12, Xiamen biomedical collaborative innovation and entrepreneurship center, 71 HouXiang Road, Haicang District, Xiamen