Protein G Sanarose 4FF
Classify :
Antibody Affinity Chromatography Medium
Keyword：
SANJI TECH
Product Features
Product Details
Product Introduction
Protein G Sanarose 4FF is an affinity medium made by immobilizing Protein G onto the Sanarose 4FF substrate through hydrogen bromide activation. Protein G has a wider binding spectrum compared to Protein A, and it not only strongly binds to antibody Fc fragments, but also weakly interacts with antibody Fab fragments. Therefore, Protein G Sanarose 4FF is commonly used for separating and purifying antibodies or antibody fragments from cell culture, as well as purifying antibodies from various species of serum.
Technical Parameter
|
Media |
Protein G Sanarose 4FF |
|
Scaffold |
Highly cross-linked 4% agarose |
|
Functional Group |
Restructure Protein G |
|
Cross-linking method |
Hydrogen bromide activation |
|
Average particle size |
90μm |
|
Tolerance to stress |
0.3Mpa |
|
Dynamic binding capacity |
>30mg human IgG/ml medium |
|
Recommended flow rates |
<300cm/h (adjusted according to column size) |
|
Chemical stability |
Tolerant to common purification buffers, 1M HAc (pH2.0), 20mM sodium phosphate, 1% SDS, 6M guanidine hydrochloride, 70% ethanol |
|
pH stability |
pH: 3~10 |
|
Save solution |
20% ethanol 2-8℃, do not freeze |
Product ordering information
filler
|
Product Name |
Article number |
Specifications |
|
Protein G Sanarose 4FF |
NPG00025 |
25ml |
|
NPG00050 |
50ml |
|
|
NPG00100 |
100ml |
|
|
NPG00250 |
250ml |
|
|
NPG00500 |
500ml |
|
|
NPG01000 |
1L |
prepacked column
|
Product Name |
Article number |
Specifications |
|
Protein G Sanarose 4FF |
NPGYZ001 |
1ml(7*25) |
|
NPGYZ05A |
5ml(16*25) |
|
|
NPGYZ05X |
5ml(8*100) |
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