Metal chelation chromatography medium can independently select chelating metal ions such as Cu ²+、 Zn ²+、 Co ²+ The main principle is that the histidine, cysteine, and tryptophan of proteins can interact with metal ions to achieve separation. IMAC Sanarose 6FF is based on Sanarose 6FF as the matrix and aminotriacetic acid as the functional ligand. Its morphology under the microscope is as follows:

CHELATING SANAROSE 6FF

Technical Parameters

Media	IMAC Sanarose 6FF
Scaffold	6% highly cross-linked agarose
Functional groups	nitrilotriacetic acid
Chelating ability	Cu²⁺: 34μmol/ml medium
Recommended flow rate	<300cm/h (adjusted according to column size)
Particle size range	45~165μm
Tolerance to stress	0.3Mpa
PH tolerance	Long term: 3-13; short term: 2-14
Chemical stability	1M NaOH, 8M urea, 6M guanidine hydrochloride
Save	20% ethanol, 4~30℃, preferably refrigerated, do not freeze, and replace the storage solution regularly.
Apply	The entire stage of purification

Product ordering information

填料

Product Name	Article number	Specifications	Chelating ability	Average particle size	Pressure resistant	Application
IMAC Sanarose 6FF	NIM00025	25ml	Ni²⁺/Zn²⁺: 15μmol/ml medium; Cu^{2 +}: 25μmol/ml medium	90μm	0.3Mpa	It is a filler that does not chelate metal ions. Customers can choose appropriate transition metal ions for chelation. The ligand is NTA (nitrilotriacetic acid). Withstand certain harsh experimental conditions.
	NIM00050	50ml
	NIM00100	100ml
	NIM00250	250ml
	NIM00500	500ml
	NIM01000	1L

Prepacked columns

product name	Article number	Specifications
IMAC Sanarose 6FF	NIMYZ001	*1ml (725)**
	NIMYZ05A	*5ml (1625)**
	NIMYZ05X	*5ml (8100)**

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