Ni-NTA Sanarose 6FF
Classify :
Histidine Labeled Protein Affinity Chromatography Medium
Keyword：
SANJI TECH
Product Features
Product Details
Product Introduction
Ni NTA Sanarose 6FF chelates nickel ions on the high flow rate agarose gel microspheres Sanarose 6FF with triacetic acid as the ligand. The bond and the four valence of metal ions make the chelated metal ions more stable, and has the advantages of withstanding higher reducing agents, good physical and chemical stability, good specificity, and high flow rate.
Ni-NTA Sanarose 6FF structural diagram
Schematic diagram of Ni-NTA Sanarose 6FF binding to His labeled protein
Technical Parameter
|
Media |
Ni-NTA Sanarose 6FF |
|
Scaffold |
Highly cross-linked 6% agarose |
|
Functional Group |
Nickel ion |
|
Ligand density |
12-18μmol/ml medium |
|
Average particle size |
90μm |
|
Tolerance to stress |
0.3Mpa |
|
Binding capacity |
>40mg/ml medium |
|
Recommended flow rates |
<300cm/h (adjusted according to column size) |
|
Chemical stability |
1M NaOH, 10mM HCl, 8M urea, 6M guanidine hydrochloride |
|
pH stability |
Long-term: 3-12 (after nickel removal); short-term: 2-14 (after nickel removal) |
|
Save solution |
20% ethanol, 4~30℃, better refrigerated, do not freeze |
|
Apply |
Large-scale purification of His tags |
Product ordering information
Filler
|
Product name |
Article number |
Specifications |
|
Ni-NTA Sanarose 6FF |
NNA00025 |
25ml |
|
NNA00050 |
50ml |
|
|
NNA00100 |
100ml |
|
|
NNA00250 |
250ml |
|
|
NNA00500 |
500ml |
|
|
NNA01000 |
1L |
Prepacked Column
|
Product name |
Article number |
Specifications |
|
Ni-NTA Sanarose 6FF |
NNYZ0001 |
1ml(7*25) |
|
NNYZ005A |
5ml(16*25) |
|
|
NNYZ005X |
5ml(8*100) |
Contact Us
About us
Address: 5th floor, building 12, Xiamen biomedical collaborative innovation and entrepreneurship center, 71 HouXiang Road, Haicang District, Xiamen