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AT Protein A Sanarose 4FF

AT Protein A Sanarose 4FF is an affinity medium synthesized by epoxidation of alkali resistant Protein A with a high flow rate agarose substrate. This medium is suitable for capturing monoclonal or Fc fusion proteins from large-scale culture media, as well as polyclonal antibodies from ascites or plasma.

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AT Protein A Sanarose 4FF

Protein G Sanarose 4FF

Protein G Sanarose 4FF is an affinity medium made by immobilizing Protein G onto the Sanarose 4FF substrate through hydrogen bromide activation. Protein G has a wider binding spectrum compared to Protein A, and it not only strongly binds to antibody Fc fragments, but also weakly interacts with antibody Fab fragments. Therefore, Protein G Sanarose 4FF is commonly used for separating and purifying antibodies or antibody fragments from cell culture, as well as purifying antibodies from various species of serum.

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Protein G Sanarose 4FF

Protein A Sanarose 4FF

Protein A Sanarose 4FF is made by coupling the cell wall protein Protein A of Staphylococcus aureus to the agarose gel matrix through epoxy chemical method. It can be widely used in the separation of monoclonal antibodies and polyclonal antibodies. It has the advantages of high load, and the ligand is not easy to fall off. The ligand protein of Protein A Sanarose 4FF has not undergone mutation modification and has limited tolerance to alkali.

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Protein A Sanarose 4FF
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