DEAE Sanarose 6FF
Classify :
Anion Exchange Chromatography Medium
Keyword：
SANJI TECH
Product Features
Product Details
Product Introduction
DEAE Sanarose 6FF is a weak anion exchange chromatography medium using Sanarose 6FF as the matrix and diethylaminoethyl as the ligand. It retains the natural hydrophilicity and porous structure of agarose, has good biocompatibility, stable physicochemical properties, can withstand strong acids and bases, and can be cleaned in place and sterilized at high temperatures. Compared with strong anion exchange chromatography media, its fully protonated pH range (working pH range) is slightly narrower and has unique separation effects.
Ion exchange chromatography technology achieves separation based on the difference in net charges on the surface of biomolecules. DEAE Sanarose 6FF is widely used in laboratories and downstream stages of biotechnology. Its operation is simple, easy to regenerate, selectivity is good, recovery rate is high, non-specific adsorption is low, and binding load is high. It can be used to remove DNA, viruses, endotoxins, acidic host cell proteins, etc. It is suitable for the initial capture or moderate purification of various scale biomolecules.
Technical Parameter
|
Media |
DEAE Sanarose 6FF |
|
Scaffold |
Highly cross-linked 6% agarose, high flow rate |
|
Media type |
Weak anion |
|
Functional group |
Diethylaminoethyl |
|
Dynamic combined loading |
110mg HSA/ml medium |
|
Average particle size |
90μm |
|
Pressure resistant |
0.3Mpa |
|
Recommended flow rate |
<300cm/h (adjusted according to column size) |
|
Working pH range |
2-12 |
|
Chemical stability |
Commonly used ion exchange buffer, 1M sodium hydroxide, 6M guanidine hydrochloride, 70%, ethanol, 30% isopropyl alcohol |
|
pH stability |
Long-term: 2-12; Short-term: 2-14; Best working pH: 2~9 |
|
Save |
20% ethanol, 4~30℃ |
|
Apply |
The entire stage of purification |
ordering information
Filler
|
Product Name |
Article number |
Specifications |
|
DEAE Sanarose 6FF |
NDB00100 |
100ml |
|
NDB00250 |
250ml |
|
|
NDB00500 |
500ml |
|
|
NDB01000 |
1L |
prepacked column
|
Product Name |
Article number |
Specifications |
|
DEAE Sanarose 6FF |
NDYZ0001 |
1ml(7*25) |
|
NDYZ005A |
5ml(16*25) |
|
|
NDYZ005X |
5ml(8*100) |
Contact Us
About us
Address: 5th floor, building 12, Xiamen biomedical collaborative innovation and entrepreneurship center, 71 HouXiang Road, Haicang District, Xiamen