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Phenyl Sanarose 6FF(LS)

Classify :

Phenyl

Keyword:

SANJI TECH


Product Features

Phenyl Sanarose 6FF (LS) is a hydrophobic chromatography medium using Sanarose 6FF as the matrix and phenyl as the functional ligand. It has the advantages of high hardness, fast flow rate, strong selectivity, high activity recovery rate, and no pollution to the environment. It can withstand strong acids and alkalis, and can be cleaned in place and sterilized at high temperatures. Compared with reverse phase chromatography medium, the ligand concentration is low and the elution conditions are mild, which can better maintain the biological activity of the sample.

Product Details


Product Introduction

Phenyl Sanarose 6FF (LS) is a hydrophobic chromatography medium using Sanarose 6FF as the matrix and phenyl as the functional ligand. It has the advantages of high hardness, fast flow rate, strong selectivity, high activity recovery rate, and no pollution to the environment. It can withstand strong acids and alkalis, and can be cleaned in place and sterilized at high temperatures. Compared with reverse phase chromatography medium, the ligand concentration is low and the elution conditions are mild, which can better maintain the biological activity of the sample.
Hydrophobic interaction chromatography is a chromatographic technique established based on the hydrophobic effects between solutes, polar mobile phases, and non polar stationary phase surfaces; In hydrophobic chromatography, binding force is mainly affected by ligand type, buffer salt, chromatography temperature, pH, and other factors. Phenyl Sanarose 6FF (LS) is widely used in the laboratory and downstream stages of biotechnology, and is commonly used for capture and moderate purification in typical three part purification strategies; It has the characteristics of "high salt absorption and low salt concentration elution" and can be combined with other separation technologies.

 

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介质

Phenyl Sanarose 6FF(LS)

基质

6%高度交联琼脂糖

配基

苯基

配基浓度

20μmol/ml胶

推荐流速

300cm/h(根据柱尺寸调整)

粒径范围

45~165μm

平均粒径

90μm

耐受压力

0.3Mpa

耐受pH

短期:3~13;工作pH:2~14

化学稳定性

常用的疏水层析缓冲液、1M氢氧化钠、6M盐酸胍、70%乙醇、1M乙酸、1M乙酸

保存

20%乙醇, 4~30℃,冷藏更佳,切勿冷冻,定期更换保存溶液。

清洗与再生

可用1M NaOH溶液浸泡1~2小时以达到灭菌效果;可根据污染物类型用1M NaOH 、70%乙醇、30%异丙醇、超纯水等冲洗多个柱体积到达清洗再生的效果。

 

 

Product ordering information

filler

产品名称

货号

规格

Phenyl Sanarose 6FF(LS)

NPL00025

100mL

NPL00100

250mL

NPL00250

500mL

NPL00500

1L

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产品名称

货号

规格

 Phenyl Sanarose 6FF(LS)

NPlYZ001

1ml7*25

NPlYZ05A

5ml16*25

NPlYZ05X

5ml8*100

 

 

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