Phenyl Sanarose 6FF(LS)
Classify :
Phenyl
Keyword:
SANJI TECH
Product Features
Product Details
Product Introduction
Phenyl Sanarose 6FF (LS) is a hydrophobic chromatography medium using Sanarose 6FF as the matrix and phenyl as the functional ligand. It has the advantages of high hardness, fast flow rate, strong selectivity, high activity recovery rate, and no pollution to the environment. It can withstand strong acids and alkalis, and can be cleaned in place and sterilized at high temperatures. Compared with reverse phase chromatography medium, the ligand concentration is low and the elution conditions are mild, which can better maintain the biological activity of the sample.
Hydrophobic interaction chromatography is a chromatographic technique established based on the hydrophobic effects between solutes, polar mobile phases, and non polar stationary phase surfaces; In hydrophobic chromatography, binding force is mainly affected by ligand type, buffer salt, chromatography temperature, pH, and other factors. Phenyl Sanarose 6FF (LS) is widely used in the laboratory and downstream stages of biotechnology, and is commonly used for capture and moderate purification in typical three part purification strategies; It has the characteristics of "high salt absorption and low salt concentration elution" and can be combined with other separation technologies.
prepacked column
介质 |
Phenyl Sanarose 6FF(LS) |
基质 |
6%高度交联琼脂糖 |
配基 |
苯基 |
配基浓度 |
20μmol/ml胶 |
推荐流速 |
300cm/h(根据柱尺寸调整) |
粒径范围 |
45~165μm |
平均粒径 |
90μm |
耐受压力 |
0.3Mpa |
耐受pH |
短期:3~13;工作pH:2~14 |
化学稳定性 |
常用的疏水层析缓冲液、1M氢氧化钠、6M盐酸胍、70%乙醇、1M乙酸、1M乙酸 |
保存 |
20%乙醇, 4~30℃,冷藏更佳,切勿冷冻,定期更换保存溶液。 |
清洗与再生 |
可用1M NaOH溶液浸泡1~2小时以达到灭菌效果;可根据污染物类型用1M NaOH 、70%乙醇、30%异丙醇、超纯水等冲洗多个柱体积到达清洗再生的效果。 |
Product ordering information
filler
产品名称 |
货号 |
规格 |
Phenyl Sanarose 6FF(LS) |
NPL00025 |
100mL |
NPL00100 |
250mL |
|
NPL00250 |
500mL |
|
NPL00500 |
1L |
prepacked column
产品名称 |
货号 |
规格 |
Phenyl Sanarose 6FF(LS) |
NPlYZ001 |
1ml(7*25) |
NPlYZ05A |
5ml(16*25) |
|
NPlYZ05X |
5ml(8*100) |