Octyl Sanarose 6FF
Classify :
Octyl Group
Keyword：
SANJI TECH
Product Features
Product Details
Product Introduction
Octyl Sanarose 6FF is a hydrophobic chromatography medium using Sanarose 6FF as the matrix and octyl as the functional ligand. It has the advantages of high hardness, fast flow rate, strong selectivity, high activity recovery rate, and no pollution to the environment. It can withstand strong acids and alkalis, and can be cleaned in place and sterilized at high temperatures. Compared with reverse phase chromatography medium, the ligand concentration is low and the elution conditions are mild, which can better maintain the biological activity of the sample.
Hydrophobic interaction chromatography is a chromatographic technique established based on the hydrophobic effects between solutes, polar mobile phases, and non polar stationary phase surfaces; In hydrophobic chromatography, binding force is mainly affected by ligand type, buffer salt, chromatography temperature, pH, and other factors. Octyl Sanarose 6FF is widely used in the laboratory and downstream stages of biotechnology, and is commonly used for capture and moderate purification in typical three part purification strategies.
Technical Parameter
|
Media |
Octyl Sanarose 6FF |
|
Matrix |
6% highly cross-linked agarose |
|
ligand |
Hinki |
|
Ligand concentration |
5μmol/ml medium |
|
Recommended flow rates |
<300cm/h (adjusted according to column size) |
|
Particle size range |
45~165μm |
|
Average particle size |
90μm |
|
Tolerance to stress |
0.3Mpa |
|
pH tolerant |
Short term: 2~14; working pH: 3~13 |
|
Chemical stability |
Commonly used hydrophobic chromatography buffer, 1M sodium hydroxide, 6M guanidine hydrochloride, 70% ethanol, 1M acetic acid. |
|
Save |
Storage: 20% ethanol, 4~30℃, preferably refrigerated, do not freeze, and replace the storage solution regularly. |
|
Cleaning and Regeneration |
It can be soaked in 1M NaOH solution for 1 to 2 hours to achieve sterilization effect; 1M NaOH, 70% ethanol, 30% isopropyl alcohol, and ultrapure water can be used according to the type of pollutants. Wait for multiple column volumes to be flushed to achieve the effect of cleaning and regeneration. |
Product ordering information
filler
|
Product Name |
Article number |
Specifications |
| Octyl Sanarose 6FF |
NOl00100 |
100mL |
|
NOl00250 |
250mL |
|
|
NOl00500 |
500mL |
|
|
NOl01000 |
1L |
prepacked column
|
Product Name |
Article number |
Specifications |
| Octyl Sanarose 6FF |
NOlYZ001 |
1mL(7*25) |
|
NOlYZ05A |
5mL(16*25) |
|
|
NOlYZ05X |
5mL(8*100) |
Contact Us
About us
Address: 5th floor, building 12, Xiamen biomedical collaborative innovation and entrepreneurship center, 71 HouXiang Road, Haicang District, Xiamen